rnase h treatment (Thermo Fisher)

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Thermo Fisher rnase h treatment
Rnase H Treatment, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnase+h+treatment/Ribonuclease+A/pmc12571865-387-17-45
Average 99 stars, based on 1 article reviews

rnase h treatment - by Bioz Stars, 2026-09

99/100 stars

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Synthesized:

Article Title: Antennal transcriptome analysis reveals sensory receptors potentially associated with host detection in the livestock pest Lucilia cuprina
Article Snippet: Subsequently, the mRNA was fragmented randomly using a fragmentation buffer (Illumina) followed by the first-strand cDNA synthesis using mRNA as a template, random hexamer primers, a custom buffer (Illumina), dNTPs (Invitrogen) and DNA polymerase I (Thermo-Fisher). .. The second strand was synthesized after a RNAse H treatment (Thermo-Fisher). .. Finally, after the sequence terminal repair, sequencing adaptors (Thermo-Fisher) were ligated.

Reverse Transcription:

Article Title: Profiling of HIV-1 elite neutralizer cohort reveals a CD4bs bnAb for HIV-1 prevention and therapy
Article Snippet: .. Reverse transcription was performed with primer YB383 5′-TTTTTTTTTTTTTTTTTTTTTTTTRAAGCAC-3′ (ref. ) and SuperScript IV (Thermo Fisher), followed by RNase H treatment (0.25 U μl −1 , 37 °C, 20 min). env cDNA was serially diluted and amplified by nested PCR using Platinum Taq Green Hot Start (Thermo Fisher) with HIV-1 YU2-NL4-3 -specific primers. ..

Article Title: Profiling of HIV-1 elite neutralizer cohort reveals a CD4bs bnAb for HIV-1 prevention and therapy.
Article Snippet: Generation of mutant HIV-1YU2 and HIV-1BG505 pseudovirus mutants Point mutations were introduced into HIV-1YU2 or HIV-1BG505 gp160 plasmids using a Q5 Site-Directed Mutagenesis kit (New England Biolabs) per the manufacturer’s protocol, and pseudovirus mutants were generated as described above. .. Reverse transcription was performed with primer YB383 5′-TTTTTTTTTTTTTTTTTTTTTTTTRAAGCAC-3′ (ref. 66) and SuperScript IV (Thermo Fisher), followed by RNase H treatment (0.25 U μl−1, 37 °C, 20 min). env cDNA was serially diluted and amplified by nested PCR using Platinum Taq Green Hot Start (Thermo Fisher) with HIV-1YU2-NL4-3-specific primers. ..

Amplification:

Nested PCR:

Incubation:

Article Title: GPATCH4 functions as a regulator of nucleolar R-loops in hepatocellular carcinoma cells
Article Snippet: .. For RNase H treatment, cells were incubated in block buffer containing 50 U/ml RNase H (Thermo Fisher Scientific) for 1 h at 37°C. .. For RNase T1 treatment, cells were incubated in block buffer containing 100 U/ml RNase T1 (Thermo Fisher Scientific) for 15 min at 37°C.

Blocking Assay:

Real-time Polymerase Chain Reaction:

Article Title: Positive effect of phasin in biohydrogen production of non polyhydroxybutyrate-producing Clostridium acetobutylicum ATCC 824.
Article Snippet: • The phaP gene was first introduced for the purpose of enhancing hydrogen

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